Light emitting diode irradiation protect against the amyloid beta 25–35 induced apoptosis of PC12 cell in vitro

✍ Scribed by Rui Duan; Ling Zhu; Timon Cheng-Yi Liu; Yan Li; Jiang Liu; Jianling Jiao; Xiaoyang Xu; Libo Yao; Songhao Liu

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 84 KB
• Volume: 33
• Category: Article
• ISSN: 0196-8092
• DOI: 10.1002/lsm.10216

✦ Synopsis

Abstract

Background and Objectives

Whether light emitting diode (LED) irradiation has effects on “β‐amyloid (Aβ) induced apoptosis,” a leading hypothesis of the cause of Alzheimer's disease (AD), or not?

Study Design/Materials and Methods

Monolayer cell cultures of PC12 were subjected to Aβ or/and LED irradiation at various intensity. Cell apoptosis was confirmed by morphological criteria, DNA fragmentation assay, and FAScan flow cytometer assay.

Results

Treatment of the cells with LED irradiation significantly diminished Aβ induced apoptosis within 24 hours.

Conclusions

The LED irradiation, when utilized at power of 0.9 W/m^2^ and 60 minutes has significantly diminished Aβ induced apoptosis of PC12 cells. Lasers Surg. Med. 33:199–203, 2003. © 2003 Wiley‐Liss, Inc.