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Ligand Binding to Polymerized Pyridoxylated Hemoglobin

โœ Scribed by Bellelli, A. ;Brunori, M. ;Giardina, B. ;Condo, S. G. ;Kothe, N. ;Bonhard, K.

Book ID
120445334
Publisher
Informa UK (Taylor & Francis)
Year
1988
Tongue
English
Weight
48 KB
Volume
16
Category
Article
ISSN
0890-5533

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Pyridoxylated adult human hemoglobin (HbAo) was prepared using a one molar equivalent of pyridoxal5-phosphate (PLP) per heme and reduced with either NaCNBH, or NaBH,. A separate sample was pyridoxylated and passed through a mixed-bed ion exchange column without reduction. All three preparations had