Ligand Binding to Macromolecules or Micelles: Use of Centrifugal Ultrafiltration to Measure Low-Affinity Binding

Low-Affinity Binding Determined by Titra

Low-Affinity Binding Determined by Titration Calorimetry Using a High-Affinity Coupling Ligand: A Thermodynamic Study of Ligand Binding to Protein Tyrosine Phosphatase 1B

✍ Yan-Ling Zhang; Zhong-Yin Zhang 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 187 KB

A competition-based method is used for the determination of the thermodynamic parameters for a low-affinity ligand binding reaction by isothermal titration calorimetry. This method is based on the coupling of a high-affinity ligand to the binding of the low-affinity ligand. Results are presented for