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Ligand Binding Assays in the 21stCentury Laboratory—A Call for Change

✍ Scribed by Ray, Chad A.; Ahene, Ago B.


Book ID
118729013
Publisher
American Association of Pharmaceutical Scientists
Year
2012
Tongue
English
Weight
100 KB
Volume
14
Category
Article
ISSN
1550-7416

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A conformational change in the lactose p
✍ Heinrich Jung; Kirsten Jung; H. Ronald Kaback 📂 Article 📅 1994 🏛 Cold Spring Harbor Laboratory Press 🌐 English ⚖ 607 KB

## Abstract Lactose transport in membrane vesicles containing lactose permease with a single Cys residue in place of Val 315 is inactivated by __N__‐ethylmaleimide in a manner that is stimulated by substrate or by a H^+^ electrochemical gradient (δμ, Sahin‐Tóth M, Kaback HR, 1993, __Protein Sci 2__