Lifespan extension of basal cell nevus syndrome fibroblasts by transfection with mouse pro or v-myc genes
✍ Scribed by T. Shimada; W. K. Dowjat; T. D. Gindhart; M. I. Lerman; N. H. Colburn
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- French
- Weight
- 888 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Dermal fibroblasts from patients with the autosomal dominant cancer-prone disease Basal Cell Nevus Syndrome (BCNS) exhibit a serum dependence, anchorage dependence and in cells vitro lifespan (about 20 population doublings or less) similar BCNS dermal fibroblast cell strains GM 2098 and GM 1575 to those of fibroblasts from normal age-, race-and Sex-and their age-, race-and sex-matched normal fibroblastic cells matched controls. Transfection with v-myc or with an acti-GM 2912 and GM 3651 were purchased from the Institute for Medical Research, Camden, NJ. A description of these cells vated mouse prml gene (which specifies sensitivity to promotion of neoplastic transformation in JB6 mouse epidermal . cells) specifically conferred partial immortality on the BCNS 1s given in Table 1. Non-tumorigenic human embryonic muscle fibroblasts by substantially extending their population dou-fibroblasts (HEM) and their tumorigenic derivatives were bling levels by more than 19 population doublings. This sug-kindly supplied by Dr. P. Fischinger of the National Cancer gests that either v-myc or pro-l gene can cooperate with Institute (Fischinger and O'Conner , 1970).
BCNS gene(s) to produce an extension of lifespan or partial
immortality. However, the transfected BCNS fibroblasts that culture Conditions escaped senescence were anchorage-dependent even after ex-Cells were initially cultured in medium containing F-12 and posure to the tumor promoters 12-0-tetradecanoyl-phorbol-Dulbecco's minimum essential medium at 1 : 1 supplemented derived growth factor (PDGF). These observations indicate that BCNS fibroblasts differ from their normal counterparts 12 weeks, medium was changed to minimum essential medium in susceptibility to extended growth and may therefore be (Eagle) supplemented with heat-inactivated fetal bovine and pre-neoplastic. It is clear that they require more than an newborn calfsera at 7.5% each (Medium II), since both BCNS actlvated pro or myc gene for progression to the tumor cell and normal fibroblasts showed better proliferation in this mephenotype.