✦ LIBER ✦

Lewis typing of human saliva stains by enzyme-linked immunosorbent assay (ELISA) using monoclonal anti-Lea and anti-Leb antibodies

✍ Scribed by Takehiko Takatori; Yoshimitsu Tsutsubuchi; Koichi Terazawa; Masataka Nagao; Hiromitsu Akabame; Hachiroh Mikami

Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 501 KB
Volume: 47
Category: Article
ISSN: 0379-0738
DOI: 10.1016/0379-0738(90)90295-a

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✦ Synopsis

The Lewis blood grouping of human saliva stains could be detected by an enzyme-linked immunosorbent assay (ELISA) using anti-Le(a) and anti-Le(b) monoclonal antibodies with an avidin-biotin complex (ABC). The saliva stains (1.0 by 1.0 cm in size) were used as samples and not only could the Lewis substances of 57 individual stains be correctly typed by this method, but also it was clarified that there are several different secretion patterns of amounts of Le(a) and Le(b) substances in 3 individual Lewis types.