Levinson DJ, silcox DC, rippon JW, and thomsen S: septic arthritis due to nonencapsulated cryptococcus neoformans with coexisting sarcoidosis.arthritis rheum 17:1037-1047, 1974

• Book ID: 101644632
• Publisher: John Wiley and Sons
• Year: 1975
• Tongue: English
• Weight: 86 KB
• Volume: 18
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/art.1780180318

✦ Synopsis

Serum M-components may be found in patients with primary amyloidosis. We recently studied such a patient (AC 72-168) with an IgG M-component who had a nephrotic syndrome and ascites. An M-component was also demonstrated in the peritoneal fluid and found by agarose electrophoresis to be identical with that of her serum. The peritoneal fluid M-component was extracted by ammonium sulfate precipitation and isolated by starch block electrophoresis. The protein was reduced, alkylated, and chromatographed on Sephadex G-100 into its heavy-and lightchain components. The resulting light chain reacted by immunodiffusion with anti-kappa BJP.

Amyloid fibrils were isolated from the patient's liver by exhaustive saline washes, denatured in 5 M guanidine, and fractionated by Sepharose 6B chromatography. The