Levels of myc protein, as analyzed by flow cytometry, correlate with cell growth potential in malignant b-cell lymphomas
✍ Scribed by Harald Holte; Trond Stokke; Erlend Smeland; Rosemary Watt; Heidi Kiil Blomhoff; Olav Kaalhus; Rolf Ohlsson
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- French
- Weight
- 846 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
We have analyzed c-myc protein expression during the cell cycle in malignant B-cell lymphomas by dual flow cytornetric detection of a fluoresceinated polyclonal anti-myc antibody and propidium iodide which binds stochiometrically to DNA. The data obtained were correlated to other parameters of cell activation such as histopathological grading, expression of the activation antigen 4F2, light scatter (proportional to cellular volume), DNA synthesis and percentage of S-phase cells. The c-myc protein level was strongly correlated to parameters of DNA synthesiskontent. In addition, the oncoprotein level was largely unvarying from the late GI phase through the rest of the cell cycle in both malignant cells and normal purified 6 cells stimulated to proliferate in virro.
' To whom reprint requests should be addressed.