Leukocyte?biomaterial interactions in the presence ofStaphylococcus epidermidis: Flow cytometric evaluation of leukocyte activation (Student Research Award in the Hospital Intern, Resident, or Clinical Fellow Category, 23rd Annual Meeting of the Society for Biomaterials, New Orleans, LA, April 30-May 4, 1997)

✍ Scribed by Sapatnekar, Suneeti ;Kao, W. John ;Anderson, James M.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 232 KB
• Volume: 35
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(19970615)35:4<409::aid-jbm1>3.0.co;2-l

✦ Synopsis

The adhesion of bacteria on a biomaterial surface significant complement activation. PMN adhesion on the biois believed to be the first step in the development of biomate-material surface was sensitive to shear stress and minimal rial-related infection. The goal of this study was to investigate at shear stress Ͼ10 dynes/cm 2 . Low concentrations of bactethe mechanisms that permit adherent bacteria to persist on ria could induce a significant increase in the expression of the surface of an implanted cardiovascular biomaterial. We PMN adhesion molecules CD11b and CD11c. We conclude hypothesized that circulating leukocytes are unable to adhere that the presence of bacteria induces PMN activation but to the biomaterial surface under physiologic shear stress con-does not increase PMN adhesion on biomaterial surfaces ditions, and this prevents them from interacting with adher-under physiologic shear stress conditions. This could be a ent bacteria. To address this hypothesis, we investigated the major mechanism that protects adherent bacteria from PMN adhesion profiles of Staphylococcus epidermidis and polymor-antibacterial activity.