✦ LIBER ✦

Leukemia-induced bone marrow depression: Effects of gangliosides on erythroid cell production

✍ Scribed by Hannie Sietsma; Willem A. Kamps; Bert Dontje; Dick Hendriks; Jan W. Kok; Edo Vellenga; Willem Nijhof

Publisher: John Wiley and Sons
Year: 1999
Tongue: French
Weight: 191 KB
Volume: 82
Category: Article
ISSN: 0020-7136
DOI: 10.1002/(sici)1097-0215(19990702)82:1<92::aid-ijc16>3.0.co;2-j

No coin nor oath required. For personal study only.

✦ Synopsis

Bone marrow depression is a common feature in hematological malignancies or other bone marrow-involving cancers. The mechanism of this hemopoietic suppression resulting in pancytopenia and especially anemia has not been elucidated. Gangliosides can be shed by cancer cells. Therefore, we investigated the effects of exogenously added gangliosides on erythropoiesis in a human and murine in vitro system. A dose-dependent inhibition of murine colony-forming-uniterythroid (CFU-E) and burst-forming-unit-erythroid (BFU-E) colony growth was observed. Furthermore the maturation of BFU-Es into CFU-Es was inhibited. The inhibition by gangliosides was not abolished by increasing the dose of erythropoietin (10 U/ml). FACS-analysis studies with human CD34 ؉ cells cultured with gangliosides (GM3) , erythropoietin (EPO) and stem cell factor (SCF) demonstrated a strong inhibition on cell growth. This resulted in a significantly higher percentage of immature cells (CD34 ؉ /GpA ؊ , 24% vs. 3%), and a lower percentage of mature erythroid cells (CD34 ؊ /GpA ؉ , 36% vs. 89%). Under these circumstances the effects on erythroid cell growth were much higher than on other cell lineages. The inhibitory effect of gangliosides isolated from acute lymphoblastic leukemic patients on in vitro erythropoiesis suggests that in vivo hemopoietic suppression might have its origin in the gangliosides present and probably shed by the malignant cells in the microenvironment and plasma. Our results show that gangliosides inhibit erythropoiesis in vitro at several stages of development, by a mechanism involving modulation of the maturation of erythroid cells.

📜 SIMILAR VOLUMES

EFFECT OF BONE MORPHOGENETIC PROTEIN-6 O

EFFECT OF BONE MORPHOGENETIC PROTEIN-6 ON HAEMOPOIETIC STEM CELLS AND CYTOKINE PRODUCTION IN NORMAL HUMAN BONE MARROW STROMA

✍ N. Ahmed; J. Sammons; R.J. Carson; M.A. Khokher; H.T. Hassan 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 118 KB

Normal human bone marrow stroma cells include stem cells for both haemopoietic and osteochondrogenic lineages and express both bone morphogenetic protein (BMP) type I and type II receptors. As a member of the TGF‐β super‐family, BMP‐6 binds to both BMP type I and type II receptors and is involved in

ChemInform Abstract: Lupane-Type Triterp

ChemInform Abstract: Lupane-Type Triterpenoids from the Steamed Leaves of Acanthopanax koreanum and Their Inhibitory Effects on the LPS-Stimulated Pro-Inflammatory Cytokine Production in Bone Marrow-Derived Dendritic Cells.

✍ Jeong Ah Kim; Seo Young Yang; Jung-Eun Koo; Young-Sang Koh; Young Ho Kim 📂 Article 📅 2011 🏛 John Wiley and Sons ⚖ 25 KB

Effects of mushroom-derived β-glucan-ric

Effects of mushroom-derived β-glucan-rich polysaccharide extracts on nitric oxide production by bone marrow-derived macrophages and nuclear factor-κB transactivation in Caco-2 reporter cells: Can effects be explained by structure?

✍ Julia J. Volman; Johannes P. F. G. Helsper; Song Wei; Johan J. P. Baars; Leo J. 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 239 KB

## Abstract Mushrooms are known for their immune‐modulating and anti‐tumour properties. The polysaccharide fraction, mainly β‐glucans, is responsible for the immune‐modulating effects. Fungal β‐glucans have been shown to activate leukocytes, which depend on structural characteristics of β‐glucans.