Leuconostoc dextransucrase and dextran: production, properties and applications
β Scribed by Myriam Naessens; An Cerdobbel; Wim Soetaert; Erick J Vandamme
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2005
- Tongue
- English
- Weight
- 240 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0268-2575
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β¦ Synopsis
Abstract
This review covers the production, properties and applications of the biopolysaccharide dextran; this biopolymer can be produced via fermentation either with Leuconostoc mesenteroides strains and other lactic acid bacteria or with certain Gluconobacter oxydans strains. The former strains convert sucrose into dextran with the dextransucrase enzyme whereas the latter convert maltodextrins into dextran with the dextran dextrinase enzyme. Emphasis is mainly focused on Leuconostoc strains as producer organisms of dextransucrase and dextran types. In addition to industrial fermentation processes producing the enzymes and/or the dextrans, biocatalysis principles are also being developed, whereby enzyme preparations convert sucrose or maltodextrins, respectively, into (oligo)dextrans. The chemical and physical properties of different dextrans are discussed in detail, together with the characteristics and molecular mode of action of dextransucrase. Subsequently, useful applications of dextran and some problems associated with undesirable formation of dextran are outlined. Copyright Β© 2005 Society of Chemical Industry
π SIMILAR VOLUMES
## Abstract Dextransucrase from __Leuconostoc mesenteroides__ (NRRL Bβ512F) was purified by ultrafiltration and gel filtration chromatography in 54% yield. The specific activity of a heart cut was 58.6 U/mg; cumulative purification of that preparation was 247βfold. Of 13 carriers surveyed, only alk
The production of dextransucrase, dextran and fructose by sucrose fermentation using Leuconostoc mesenteroides NRRL-B512(F) was studied in batch operation in a bioreactor with total working volume of 1.5 dm 3 . The effect of temperature (20 to 40 β’ C), pH (5.5 and 6.7) and sucrose concentration (10