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Letter to the Editor: NMR-based solution structure of the complex ofLactobacillus caseidihydrofolate reductase with trimethoprim and NADPH

✍ Scribed by Vladimir I. Polshakov; Eugeni G. Smirnov; Berry Birdsall; Geoff Kelly; James Feeney


Book ID
110398070
Publisher
Springer Netherlands
Year
2002
Tongue
English
Weight
156 KB
Volume
24
Category
Article
ISSN
0925-2738

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3D13C/1H NMR-based assignments for side-
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~3C-based three-dimensional 1H-1H correlation experiments have been used to determine essentially complete 13C and 1H resonance assignments for the amino acid side chains of uniformly 13C/15N labelled L. casei dihydrofolate reductase in a complex with the drug methotrexate. Excellent agreement is ob