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Letter to the Editor: High-resolution separation of PCR product and gene diagnosis by capillary gel electrophoresis

✍ Scribed by Alexandra Schlenck; Sophia Visvikis; Maurice O'Kane; Gérard Siest

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
187 KB
Volume
10
Category
Article
ISSN
0269-3879

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✦ Synopsis

In a recent issue of the journal, Baba er al. (1994), demonstrate the high resolution and the high-speed gene diagnostic power of Capillary gel electrophoresis (CGE) through human apolipoprotein E genotyping (PCR amplification of DNA and HhaI restriction enzymatic cleavage (Hixson and Vernier, 1990)). They demonstrate the high potential of CGE for PCR product analysis. It is clear that improvement in resolution, speed (Pariat et af., 1993;), quantitation (Butler et al., 1994), sensitivity (Cord et al., 1993), reproducibility (Butler ef al., 1994) and automation are among the characteristics that CGE offers relative to electrophoresis in the conventional slab gel format. However the results provided by Baba and coworkers are incomplete Author to whom correspondence should be addressed. and further work is required to demonstrate the role of CGE in the study of apoE genotype.

📜 SIMILAR VOLUMES

High-resolution separation of PCR produc
High-resolution separation of PCR product and gene diagnosis by capillary gel electrophoresis
✍ Yoshinobu Baba; Riyo Tomisaki; Chinuyo Sumita; Mitsutomo Tsuhako; Tetsuro Miki; 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 262 KB

High-resolution separation of a PCR product from a mixture of DNA restriction fragments was achieved using capillary gel electrophoresis. The capillary gel electrophoretic separation gives an excellent resolution of two fragments of the 500-bp PCR product and the 506-bp DNA fragment, which differs b