LETTER TO THE EDITOR. Cross-hybridization of the chromosome 13/21 alpha satellite DNA to chromosome 22 or a rare polymorphism?

✍ Scribed by E. P. TARDY; A. TÓTH

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 453 KB
• Volume: 17
• Category: Article
• ISSN: 0197-3851
• DOI: 10.1002/(sici)1097-0223(199705)17:5<487::aid-pd81>3.0.co;2-q

✦ Synopsis

Cross-hybridization of the chromosome 13/21 alpha satellite DNA to chromosome 22 or a rare polymorphism?

Verlinsky et al. reported a false-positive diagnosis due to a cross-hybridization of chromosome 13/21 alpha satellite DNA to chromosome 22 in a prospective large-scale study in Prenatal Diagnosis in Volume 15, pp. 831-834. On behalf of the probe supplier Oncor, Mr J. K. Blancato reflected on this article in Prenatal Diagnosis in Volume 16, p. 769, together with the authors' reply. We would like to share our findings in a similar case to provide more data on this phenomenon.

Our patient, a 4-year-old boy, was referred for cytogenetic investigation in 1994 because of mild mental retardation and slight dysmorphic features. The conventional cytogenetic result was 46,XY. As being normal, his lymphocyte suspension was blindly selected from our stored archival material for setting up FISH protocols for interphase