Background: Human peripheral blood lymphocytes kept in culture after isolation die by an apoptotic process. Detection of apoptosis with labeled Annexin V to demonstrate loss of plasma membrane asymmetry is sensitive, specific, and easy using flow cytometry. This is true in lymphoblastic cell lines w
β¦ LIBER β¦
Letter: Flow cytometric study of T-cell subsets in lymphocytic pleural effusions
β Scribed by Rasha Y. Khalil; Madgy M. Khalil
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 30 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0196-4763
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Expression of the T-cell receptor (TCR) zeta chain in normal individuals was studied by two-color flow cytometric analysis using digitonin-permeabilized human peripheral blood lymphocytes. Optimal detection of the TCR zeta chain involved fixation of cells in 0.25% paraformaldehyde for 2 min and perm