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Leptin regulates estrogen receptor gene expression in ATDC5 cells through the extracellular signal regulated kinase signaling pathway

✍ Scribed by Shan-Jin Wang; Xin-Feng Li; Lei-Sheng Jiang; Li-Yang Dai

Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
398 KB
Volume
113
Category
Article
ISSN
0730-2312

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✦ Synopsis

Abstract

Both estrogen and leptin play an important role in the regulation of physiological processes of endochondral bone formation in linear growth. Estrogen receptors (ERα and ERβ) are known as members of the superfamily of nuclear steroid hormone receptors and are detected in all zones of growth plate chondrocytes. They can be regulated in a ligand‐independent manner. Whether leptin regulates ERs in the growth plate is still not clear. To explore this issue, chondrogenic ATDC5 cells were used in the present study. Messenger RNA and protein analyses were performed by quantitative PCR and Western blotting. We found that both ERα and ERβ were dynamically expressed during the ATDC5 cell differentiation for 21 days. Leptin (50ng/ml) significantly upregulated ERα and ERβ mRNA and protein levels 48 h after leptin stimulation (P < 0.05) at day 14. The up‐regulation of ERα and ERβ mRNA by leptin was shown in a dose‐dependent manner, but the most effective dose of leptin was different (100 and 1,000 ng/ml, respectively). Furthermore, we confirmed that leptin augmented the phosphorylation of ERK1/2 in a time‐dependent manner. A maximum eightfold change was observed at 15 min. Finally, a specific ERK1/2 inhibitor, UO126, blocked leptin‐induced ERs regulation in ATDC5 cells, indicating that ERK1/2 mediates, partly, the effects of leptin on ERs. These data demonstrate, for the first time, that leptin regulates the expression of ERs in growth plate chondrocytes via ERK signaling pathway, thereby suggesting a crosstalk between leptin and estrogen receptors in the regulation of bone formation. J. Cell. Biochem. 113: 1323–1332, 2012. © 2011 Wiley Periodicals, Inc.

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