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Leishmania Promastigote Membrane Antigen-Based Enzyme-Linked Immunosorbent Assay and Immunoblotting for Differential Diagnosis of Indian Post-Kala-Azar Dermal Leishmaniasis

✍ Scribed by Saha, S.; Mazumdar, T.; Anam, K.; Ravindran, R.; Bairagi, B.; Saha, B.; Goswami, R.; Pramanik, N.; Guha, S. K.; Kar, S.; Banerjee, D.; Ali, N.

Book ID: 121532986
Publisher: American Society for Microbiology
Year: 2005
Tongue: English
Weight: 452 KB
Volume: 43
Category: Article
ISSN: 0095-1137
DOI: 10.1128/JCM.43.3.1269-1277.2005

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For immunodiagnosis of kala-azar enzyme-linked immunosorbent assay (ELISA) and immunofluorescence testing (IFAT) are commonly used. In IFAT, whole parasite antigen and in ELISA the soluble antigen have been used. Preparation of ELISA antigen has certain inherent difficulties. We have developed a sim