Left-handed DNA in restriction fragments and a recombinant plasmid
✍ Scribed by Kłysik, Jan; Stirdivant, Steven M.; Larson, Jacquelynn E.; Hart, Phillip A.; Wells, Robert D.
- Book ID
- 109722721
- Publisher
- Nature Publishing Group
- Year
- 1981
- Tongue
- English
- Weight
- 725 KB
- Volume
- 290
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/290672a0
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
The difficulty experienced in the shotgun cloning of chromosomal DNA on plasmid vectors in Bacillus subtilis is analyzed and an explanation for this difficulty is offered based on an inherent property of competent cells which imposes a requirement of plasmid multimers in transformation of plasmid-fr
The restriction endonuclease BglI produces different individual fragment ends from different cut sites. This property has allowed us to reconstruct efficiently several commonly used plasmid and bacteriophage genomes and a number of recombinant plasmids containing up to seven BglI restriction sites f