Lectinocytochemical detection of apoptotic murine leukemia L1210 cells
✍ Scribed by R. O. Bilyy; R. S. Stoika
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 244 KB
- Volume
- 56A
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Abstract A glycoprotein extract prepared from the plasma membranes of L1210 cells was passed over columns of Sepharose 4B to which either heat‐aggregated human IgG or F(ab′)~2~ fragments had been coupled. The intact IgG column bound 35.7% of the applied counts, whereas the F(ab′)~2~ columns boun
## Abstract L1210 murine leukemia cells after treatment with __Cl. perfringens__ neuraminidase at pH 7.0 incorporated six times more N‐acetylneuraminic acid‐[C^14^] than control cells when incubated for 30 minutes with cytidine 5′‐monophosphate N‐acetylneuraminic‐[C^14^] acid and three times more g
The murine leukemia L12lO-specific cytotoxic T-cell clone K7 was established by repeated antigenic stimulation of spleen cells of LIZIO-immune CDZF, mice in long-term culture. K7 possessed L 1210-specific cytolytic activity detectable by the slCr-release test, but lost its cytolytic activity about 1
## Abstract Intact murine L1210 leukemic cells incorporated significant quantities of [^3^H]‐N‐acetylneuraminic acid directly from CMP‐N‐acetylneuraminic acid. When pretreated with Vibrio cholerae neuraminidase, incorporation increased sixfold to tenfold. Biochemical studies comparing incorporation