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Lectin-resistant variants and revertants of mouse melanoma cells: Differential expression of a fucosylated cell-surface antigen and altered metastasizing capacity

✍ Scribed by Jukka Finne; Serenella Castori; Ten Feizi; Max M. Burger

Publisher
John Wiley and Sons
Year
1989
Tongue
French
Weight
742 KB
Volume
43
Category
Article
ISSN
0020-7136

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✦ Synopsis

In order to evaluate the possible role of cell-surface carbohydrates in metastasis of tumour cells, 2 wheat-germ agglutinin-resistant (WGA') variants of 816 mouse melanoma and 8 back revertants selected with other lectins were analyzed with respect to the surface expression of fucosylated carbohydrate antigens and their metastasizing capacity. The variant cells, expressing a greatly increased fucosyltransferase activity, were found to express the fucose-containing SSEA-I antigen on their cell surface. The revertant cells selected for lower fucosylation with Lotus tetragonolobus lectin and rich had lost this particular antigen. Seven of the 8 revertant lines also reverted back to a state of increased metastasizing capacity as compared to the WGA' variants they were derived from. A single one of the revertants displayed reduced metastasizing capacity, suggesting that additional changes can also be present in some of the cell lines. These results suggest a possible linkage between expression of the developmental SSEA-I antigen and reduced metastasizing capacity in the mouse melanoma model.

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