Lectin receptor proximity on HL-60 leukemia cells determined by fluorescence energy transfer using flow cytometry
✍ Scribed by Diane M. Jenis; Anita L. Stepanowski; Owen C. Blair; Douglas E. Burger; Alan C. Sartorelli
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 736 KB
- Volume
- 121
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Fluorescence energy transfer using flow cytometric measurements was utilized to determine the proximity of concanavalin A receptors on the surface of HL-60 prornyelocytic leukemia cells before and after induction of differentiation. The HL-60 cells were induced to differentiate into granulocytes using dimethylsulfoxide and into macrophages using 12-O-tetradecanoylphorbol-13- acetate. Concanavalin A was labeled with either fluorescein (donor chromophore) or tetramethylrhodamine (acceptor chromophore), and these species were used to determine lectin proximity. With granulocytic differentiation, the amount of concanavalin A bound remained constant, but a decrease in receptor density was observed. During rnacrophage differentiation, however, both receptor density and receptor number increased. The increase in concanavalin A binding during differentiation appears to be a result of maturation rather than an initiating event.