Rabbit antibodies against rat thymus and liver chromatin are obtained. Antithymus IgG are found to interact only with homologous chromatin, while antiliver IgG interact with both liver and thymus chromatin. After preincubation of antiliver IgG with thymus chromatin the antibodies interact with homol
Lectin activity of chromatin non-histone proteins
✍ Scribed by Ljiljana Ševaljević; Miroslav Konstantinović; Milena Tomović; Zorica Pavlović
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 452 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0301-4851
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✦ Synopsis
Non-histone proteins from chromatin of sea urchin embryos were found to possess the ability to agglutinate erythrocytes.
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Three antisera were prepared against non-histone protein classes named NHCP1, NHCP2 and dehistonized chromatin (with different affinity to DNA) from hamster liver. Two main antigenic bands of MW 17,000 and 36,000 were specific in the NHCP1 fraction and one antigen of MW 56,000 was specific for the N
Non-histone proteins of normal, non-immunized rats and rats immunized with mouse spleen cells were labelled with three different amino acids: [3H]tryptophan, [3H]methionine and [3H]leucine. Chromatin was fractionated at increasing salt concentrations into three fractions: 0.35 M NaCl-soluble, 2 M Na