𝔖 Bobbio Scriptorium
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LEC–BiFC: a new method for rapid assay of protein interaction

✍ Scribed by Lin, J; Wang, N; Li, Y; Liu, Z; Tian, S; Zhao, L; Zheng, Y; Liu, S; Li, S; Jin, C; Xia, B

Book ID
119954952
Publisher
Informa plc
Year
2010
Tongue
English
Weight
731 KB
Volume
86
Category
Article
ISSN
1052-0295

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✍ Kuo-Ping Huang; J.C. Robinson 📂 Article 📅 1976 🏛 Elsevier Science 🌐 English ⚖ 421 KB

A simplified assay method is described for the determination of protein kinase activity. Enzymatic activity is followed by measuring the incorporation of 32P from the terminal phosphoryl group of nucleoside triphosphates into protein substrate. Separation of the resulting 32P-labeled phosphoprotein