LC method for the analysis of kanamycin residue in swine tissues using derivatization with 9-fluorenylmethyl chloroformate
✍ Scribed by Yiqiang Chen; Qian Chen; Shusheng Tang; Xilong Xiao
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 198 KB
- Volume
- 32
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
A sensitive and robust HPLC method was developed for the determination of kanamycin (Kan) in swine tissues. Kan was extracted from tissue with 10% TCA (w/v) solution and cleaned up with MCX SPE column. The purified extract was derivatized with 9‐fluorenylmethyl chloroformate, and then separated on a C18 column and detected by a fluorescence detector. The linear range of the calibration curve was 0.025–1 μg/mL with R^2^ of 0.9998. The limits of quantification were determined to be 0.1 mg/kg in muscle, 0.2 mg/kg in liver, and 0.6 mg/kg in kidney, and the average recoveries of Kan from swine tissues at different fortification levels (0.1–0.4 mg/kg for muscle, 0.2–1.2 mg/kg for liver, and 0.6–5.0 mg/kg for kidney) ranged from 80.7 to 91.3% with intraday and interday coefficient of variations less than 12.1%. The validated method was successfully applied to determine Kan in incurred samples, indicating that it can be used as a routine tool for the surveillance of Kan residue in swine tissues.