LC Determination of Thiols Derivatized with 4-(Aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole after SPE
โ Scribed by Ke-Jing Huang; Cong-Hui Han; Jing Li; Zhi-Wei Wu; Yan-Ming Liu; Ying-Ying Wu
- Book ID
- 106317161
- Publisher
- Springer
- Year
- 2011
- Tongue
- English
- Weight
- 199 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0009-5893
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๐ SIMILAR VOLUMES
The fluorescent cysteine derivative formed upon alkylation of proteins with 4 -(aminosulfonyl)-7-fluoro2,1,3-benzoxadiazole (ABD-F) is shown to be unstable under certain conditions. Although previously thought to be an irreversible reaction, the fluorescence and absorbance associated with the ABD-cy
The simultaneous determination of amino acid composition including cysteine of egg albumin, a model protein containing a/s cysteine residue, is reported. All the thiol groups of the cysteine residue@) of egg albumin were labelled with 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole, a fluorogenic re