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LBA technique in the detection of chromosome variants

✍ Scribed by Y. Nakagome; S. Oka; E. Matsunaga


Publisher
Springer
Year
1977
Tongue
English
Weight
446 KB
Volume
38
Category
Article
ISSN
0340-6717

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✦ Synopsis


In Part I of this communication, a technique (LBA) was described which used DNA replication in the evaluation of chromosome variants in man. It was shown that the method was very useful in the detection of variants in D- and G-group chromosomes. Results on pars 3 and 4 were also presented. In Part II, the rest of chromosomes were examined. In the evaluation of qh variants in 1, 9 and 16, the LBA technique proved itself to be a very effective implement. It was practically free of technical variables coherent with C-band technique and, therefore, it was possible to use the size of an euchromatic segment of a chromosome as a reference standard. LBA variants were observed in about 50% of the members of the remaining 12 pairs of chromosomes, i.e., 2, 5, 6, 7, 8, 10, 11, 12, 17, 18, 19, and 20.


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✍ S. Oka; Y. Nakagome; E. Matsunaga; M. Arima πŸ“‚ Article πŸ“… 1977 πŸ› Springer 🌐 English βš– 439 KB

A method is described (LBA method) which used DNA replication pattern in the detection of chromosome variants in man. In Part I, results on chromosomes with known sites of Q-variants, i.e., 5 pairs of acrocentrics, as well as 3 and 4, were presented. Fourty-one variants were detected in a total of 4

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