Layer-by-Layer Buildup of Poly(L-glutamic acid)/Chitosan Film for Biologically Active Coating

✍ Scribed by Zhijiang Song; Jingbo Yin; Kun Luo; Yanzhen Zheng; Yan Yang; Qiong Li; Shifeng Yan; Xuesi Chen

• Book ID: 102473787
• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 447 KB
• Volume: 9
• Category: Article
• ISSN: 1616-5187
• DOI: 10.1002/mabi.200800164

✦ Synopsis

Abstract

A new biocompatible film based on chitosan and poly(L‐glutamic acid) (CS/PGA), created by alternate deposition of CS and PGA, was investigated. FT‐IR spectroscopy, UV‐vis spectroscopy and QCM were used to analyze the build‐up process. The growth of CS and PGA deposition are both exponential to the deposition steps at first. After about 9 (CS/PGA) depositions, the exponential to linear transition takes place. QCM measurements combined with UV‐vis spectra revealed the increase in the multilayer film growth at different pH (4.4, 5.0 and 5.5). The build‐up of the multilayer stops after a few depositions at pH = 6.5. A muscle myoblast cell (C2C12) assay showed that (CS/PGA)~n~ multilayer films obviously promote C2C12 attachment and growth.

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