Lateral and rotational diffusion of EGF–receptor complex: Relationship to receptor-mediated endocytosis

✍ Scribed by Joseph Schlessinger

• Publisher: Wiley (John Wiley & Sons)
• Year: 1983
• Tongue: English
• Weight: 410 KB
• Volume: 22
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.360220145

✦ Synopsis

The clustering and internalization of epidermal growth factor (EGF)-receptor complexes on human tumor cells are temperature-sensitive processes that are completely inhibited a t 4°C. The rotational diffusion of EGF-receptor is limited by the viscosity of the lipid matrix, while additional constraints limit the lateral diffusion of the receptor molecule. Although the lateral and rotational diffusion of the occupied receptors are temperature sensitive, they do not provide a rate-determining factor for the clustering and subsequent internalization of the EGF-receptor. Even at 4°C the occupied receptors move quickly enough to allow the formation of EGF-receptor clusters. The lack of internalization at 4°C must be due to the inhibition of interactions other than a temperature effect on receptor mobility. EGF-induced receptor clustering involves the formation of microclusters composed of 10-50 receptor molecules.