𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Laser photobleaching of human serum: Application to fluorescence immunoassays

✍ Scribed by Abraham H. Parola; Egidijus E. Uzgiris

Book ID
102629417
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
320 KB
Volume
138
Category
Article
ISSN
0003-2697

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✦ Synopsis

Background fluorescence from serum chromophores is substantially reduced by a laser photobleaching method. Human and bovine serum samples were illuminated with 337-nm light from a pulsed N2 laser for a short period of time. The serum emission in the region of 440 to 550 nm was reduced by an order of magnitude with no evident damage to serum proteins as judged by the unchanged activity of alkaline phosphatase and aspartate aminotransferase.

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## Abstract The diffusion coefficient, __D__, and the proportion of mobile molecules, __p__, is measured for a fluorescently labeled polyelectrolyte in a multilayer film using fluorescence recovery after photobleaching (FRAP). The film was composed of poly(L‐lysine) (PLL) and hyaluronan (HA). The l