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Largest contour segmentation: A tool for the localization of spots in confocal images

✍ Scribed by E. M. M. Manders; R. Hoebe; J. Strackee; A. M. Vossepoel; J. A. Aten

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 803 KB
Volume: 23
Category: Article
ISSN: 0196-4763
DOI: 10.1002/(sici)1097-0320(19960101)23:1<15::aid-cyto3>3.0.co;2-l

No coin nor oath required. For personal study only.

✦ Synopsis

An accurate determination of the 3-D positions of multiple spots in images obtained by confocal microscopy is essential for the investigation of the spatial distribution of specific components or processes in biological specimens. The position of the centroid, as an estimator for the position of a spot, can be calculated on the basis of all voxels that belong to the domain of the spot. For this calculation a domain that defines which voxels belong to the spot must be delimited. To create a boundary for a domain we developed a 3-D image segmentation procedure: the largest contour segmentation (LCS). This procedure

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