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Large scale transient expression with COS cells

✍ Scribed by Horst D. Blasey; Jean-Pierre Aubry; Gonzalo J. Mazzei; Alain R. Bernard

Publisher
Springer
Year
1996
Tongue
English
Weight
647 KB
Volume
18
Category
Article
ISSN
0920-9069

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## Abstract Transient expression of recombinant proteins in mammalian cell culture in a 100‐L scale requires a large quantity of plasmid that is very labour intensive to achieve with shake flask cultures and commercially available plasmid purification kits. In this paper we describe a process for p