Large scale synthesis of dinucleoside monophosphates catalyzed by ribonuclease from Aspergillus clavatus
✍ Scribed by S. Bauer; R. Lamed; Y. Lapidot
- Publisher
- John Wiley and Sons
- Year
- 1972
- Tongue
- English
- Weight
- 437 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
A gram scale enzymatic synthesis of eight, dinucleoside monophosphates (ApC, ApU, CpC, CpU, GpC, GpU, UpC, and UpU) is described. The synthesis involves a reaction between the appropriate ribonucleoside‐2′,3′‐cyclie phosphates and cytidine or uridine in the presence of ribonuelease from Aspergillus clavatus at 30°C. The enzyme is removed from the reaction mixture by chromatography on Bio‐Gel P–4, and the dinucleoside monophosphate is further purified by chromatography on a DEAE‐Sephadex A–25, column.
A procedure for the large scale preparation of the ribonuclease from Aspergillus clavatus is also described.