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Large-Scale Sequencing Library Production: An Adapter-Based Strategy

โœ Scribed by C.M. Povinelli; R.A. Gibbs

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
854 KB
Volume
210
Category
Article
ISSN
0003-2697

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๐Ÿ“œ SIMILAR VOLUMES

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โœ B. Andersson; C.M. Povinelli; M.A. Wentland; Y. Shen; D.M. Muzny; R.A. Gibbs ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 671 KB

An improved strategy for the preparation of libraries for the random sequencing of DNA is reported. The protocol is a modification of a previous adaptor-based strategy, and utilizes long (11 base) overhangs, which eliminates the unreliable step of vector-insert ligation. The random inserts are prepa