✦ LIBER ✦
Large-scale separation of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza by pH-zone-refining countercurrent chromatography
✍ Scribed by Xiao Wang; Yanling Geng; Fuwei Li; Jianhua Liu
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 445 KB
- Volume
- 30
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
pH‐Zone‐refining countercurrent chromatography was successfully applied to the separation of salvianolic acid B from the Chinese medicinal plant, Salvia miltiorrhiza Bunge, using a multilayer coil planet centrifuge. A 2.0 g quantity of sample was separated using the following two‐phase solvent system: methyl tert‐butyl ether (MtBE)–water, 10 mM TFA in organic stationary phase and 10 mM ammonia in aqueous mobile phase. The obtained fractions were analyzed by HPLC and ESI‐MS. The separation yielded 572 mg of the main component of salvianolic acid B with a purity of 94.1%.