## Abstract The use of lentiviral vectors as gene delivery vehicles has become increasingly popular in recent years. The growing interest in these vectors has created a strong demand for large volumes of vector stocks, which entails the need for scaleable vector manufacturing procedures. In this wo
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Large-scale purification of halophilic enzymes by salting-out mediated chromatography
β Scribed by W. Leicht; S. Pundak
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 497 KB
- Volume
- 114
- Category
- Article
- ISSN
- 0003-2697
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A rapid procedure for the large-scale purification of pig heart nucleoside diphosphate kinase is described. The purification procedure involves extraction of the enzyme, absorption on Cibacron Blue 3G-A Sepharose, elution with ATP, ammonium sulfate precipitation, heat treatment, and rechromatography