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Large-scale purification of fungal glucoamylases using anion-exchange resin chromatography

✍ Scribed by Resham S. Bhella; Illimar Altosaar

Publisher: Elsevier Science
Year: 1984
Tongue: English
Weight: 241 KB
Volume: 140
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(84)90154-4

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✦ Synopsis

Anion-exchange chromatography on polystyrene resin is shown to be more effective than DEAE-cellulose for purification of glucoamylase from crude enzyme extracts of Aspergillus awamori or from commercial preparations. The glucoamylase from A. awamori culture medium was purified to electrophoretic homogeneity with yields approaching 80%.

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