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Large-Scale Production and Cultivation of Hepatocytes on Biosilon Microcarriers

✍ Scribed by Alexander Shnyra; Alexander Bocharov; Natalia Bochkova; Valentin Spirov

Book ID
115310238
Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
879 KB
Volume
14
Category
Article
ISSN
0160-564X

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Cultivation of the new immortalized hepatocyte cell line HepZ was performed with a 1:1 mixture of DMEM and Ham's F12 media containing 5% FCS. The cells were grown in their 40th passage in 100 mL and 1 L volumes in spinner flasks and in a bioreactor, respectively. For the production of adherently gro