Large-scale preparation of a lectin from sunn hemp seeds (Crotalaria juncea)
✍ Scribed by Bo Ersson
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 468 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Abstract
A procedure for large‐scale preparation of a lectin from Crotalaria juncea seeds is described. The method involve fractionation by pH‐ and ammonium sulfate precipitation followed by biospecific affinity chromatography. The adsorbent used for the affinity chromatography was prepared by coupling galactose to Sepharose 6B activated with divinylsulfone. A comparison of different apparatus and techniques involved in the preparation is discussed. The yield and quality of the lectin prepared at a large scale were comparable with laboratory‐scale preparation. From 50 kg Crotalaria juncea beans, 14.4 g Crotalaria lectin were obtained.