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Large-scale phosphosite quantification in tissues by a spike-in SILAC method

✍ Scribed by Monetti, Mara; Nagaraj, Nagarjuna; Sharma, Kirti; Mann, Matthias

Book ID
111657622
Publisher
Nature Publishing Group
Year
2011
Tongue
English
Weight
653 KB
Volume
8
Category
Article
ISSN
1552-4450

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A one-vial procedure has been developed to quantify free fatty acids in human blood serum and rat heart tissue. To allow routine analysis the system has been constructed to allow simultaneous processing of nine samples (Praechromat). The free fatty acids are extracted with Freon 11 and then derivati