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Large-scale isolation of phytochrome from oat seedlings

✍ Scribed by H.H. Kroes; A. Van Rooijen; Joke M. Geers; E.H.M. Greuell


Book ID
115748193
Publisher
Elsevier Science
Year
1969
Weight
281 KB
Volume
175
Category
Article
ISSN
0005-2795

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Partial purification of sequestered part
✍ E. Hofmann; R. Grimm; K. Harter; V. Speth; E. SchΓ€fer πŸ“‚ Article πŸ“… 1991 πŸ› Springer-Verlag 🌐 English βš– 990 KB

Sequestered particles of phytochrome (SAPs) were partially purified from red-light-irradiated oat coleoptiles. Phytochrome pelletability was enhanced by using buffers containing 10 mM Mg 2+ or high concentrations (0.6-0.8 M) of orthophosphate (Pi). Combining the pelletability of phytochrome in the p