Large-scale isolation and partial purification of type C RNA viruses on hydroxyapatite: 1. Biochemical characterization
✍ Scribed by Richard Guy Smith; Sin A. Lee
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 636 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
A chromatographic procedure utilizing common laboratory equipment and based on the batchwise adsorption of type C RNA virus onto hydroxyapatite for the concentration and partial purification of viruses from large volumes of tissue culture fluid has been developed. This procedure provides an alternative to the use of elaborate and expensive high-speed zonal ultracentrifuge equipment. The viruses obtained by this procedure have a buoyant density of 1.16 g/cm", contain 70 S RNA, an RNA-directed DNA polymerase (reverse transcriptase), surface glycoproteins (GP69/71), and the internal viral specific polypeptides p10 to 15 and p27 or ~30.