A new and highly sensitive assay for the
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Henry M. Kronenberg; Tikvah Vogel; Robert F. Goldberger
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Article
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1975
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Elsevier Science
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English
⚖ 500 KB
A new method is described for assaying the ATP phosphoribosyltransferase (EC 1.4.2.17) that catalyzes the first step of histidine biosynthesis in bacterial cells. This is a highly sensitive radioassay, capable of detecting fewer than lOy molecules of enzyme. In contrast to the previously available a