Laminar restriction of retinal macrophagic response to optic nerve axotomy in the rat

✍ Scribed by Garcia-Valenzuela, E. ;Sharma, S. C.

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 528 KB
• Volume: 40
• Category: Article
• ISSN: 0022-3034
• DOI: 10.1002/(sici)1097-4695(199907)40:1<55::aid-neu5>3.0.co;2-e

✦ Synopsis

Following optic nerve transection, most of the retinal ganglion cells die. Their debris is promptly cleared by phagocytic cells. It is currently not known to what extent peripherally derived macrophages contribute to this activity. Using antibodies OX42 and ED-1, phagocytic cells were labeled in the retinas of optic nerve lesioned adult rats. To distinguish whether the cells were reactive microglial or macrophagic in origin, blood-borne monocytes were labeled with fluorescent microspheres while in the systemic circulation. Macrophages invaded the retina, but only in the nerve fiber layer, sparing the ganglion cell and other layers. These macrophages engulfed only the axonal debris from dying ganglion cells, not their degenerating cell bodies. These results indicate that although peripherally derived monocytic cells are recruited into the retrogradely degenerating retina, their role in clearing debris is limited to the optic fiber layer.