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Lambda encodes an outer membrane protein: The lom gene

โœ Scribed by Reeve, John N. ;Shaw, Jocelyn E.


Book ID
104695738
Publisher
Springer
Year
1979
Tongue
English
Weight
588 KB
Volume
172
Category
Article
ISSN
0026-8925

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โœฆ Synopsis


lambda infected minicells synthesize a polypeptide (M(r) = 20,500) which is incorporated almost exclusively into the outer membrane of the minicell envelope. The gene (lom = lambda outer membrane) encoding this polypeptide has been mapped in the non-essential region of the lambda genome between coordinates 39.4% and 40.7% of lambda.


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The regulatory region of the ompA gene from Escherichia coli has been characterized by biochemical and genetic approaches. Two overlapping promoters, P1 and P2, organized in that order with respect to the ompA coding sequence, were identified and it was found that ompA possesses an unusually long le