Lactone inhibition of the β-glucosidase contaminant of an enzymatic reagent used for glucose assays

Development of an enzymatic method for t

Development of an enzymatic method for the assay of serum magnesium using phosphoglucomutase and glucose-6-phosphate dehydrogenase

✍ Kenji Suzuki; Yuzo Kayamori; Yoshiaki Katayama 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 353 KB

We developed an assay for serum magnesium (Mg) by coupling phosphoglucomutase (EC 2.7.5.1) with glucose-6-phosphate dehydrogenase (EC 1.1.1.49). The kinetic generation of NADPH by the action of the above two enzymes upon glucose-l-phosphate (Gl-P) and glucoso-l,6-diphosphate (G-1,6-P) was proportion