Lactic dehydrogenase (LDH) and transaminase (GOT) activity of synovial fluid and serum in rheumatic disease states, with a note on synovial fluid LDH isozymes
✍ Scribed by Alan S. Cohen
- Publisher
- John Wiley and Sons
- Year
- 1964
- Tongue
- English
- Weight
- 709 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0004-3591
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✦ Synopsis
Lactic acid dehydrogenase (LDH) and glutamic-oxalacetic transaminase determinations were carried out on the serums and synovial fluids of patients with a variety of rheumatic diseases. The LDH activity of the synovial fluid was elevated in patients with rheumatoid arthritis, infectious arthritis and gout, but normal in the fluids of patients with degenerative joint disease. Transaminase activity was normal in both serum and synovial fluid in all cases. Starch gel zymograms of LDH showed qualitatively increased amounts of the isozyme LDH 5 in the pathological synovial fluids.
S. COHEN
Determinationes del dehydrogenase de acido lactic e de transaminase glutamicoxaloacetic esseva executate in le seros e le liquidos synovial de patientes con un varietate de morbos rheumatic. Le activate de dehydrogenase lactic del liquido synovial esseva elevate in patientes con arthritis rheumatoidee, con arthritis infectiose, e con gutta, sed ill0 esseva normal in le liquido synovial de patientes con morbo degenerative del articulationes. Le activitate de transaminase glutamic-oxaloacetic esseva normal in omne casos tanto in le liquido synovial como etiam in le sero del sanguine. Zymogrammas a gel de amylo pro dehydrogenase de acido lactic monstrava augmentos qualitative del quantitates del isozyma 5 de hydrogenase de acido lactic.
HE VALUE of synovial fluid analysis in the differential diagnosis of T various rheumatic diseases has become increasingly apparent in the past 10 years since the publication of the definitive monograph of Ropes and Bauer in 1952.132 Cultures, white blood cell and differential counts, sugar and mucin determinations have become accepted as the most useful laboratory studies of synovial fluid. In recent years, as our knowledge of the specific physiologic activity of the syriovial membrane has i n ~r e a s e d ~, ~ data have appeared in the medicaI literature that suggest that an evaluation of the synovial 0uid enzyme content might also prove to be of significance. A number of these enzymes have been studied including amylase, lipase, alkaline phosphatase,l aminotripeptidase? beta glucuronidase,e and others.7 Until recently, only limited data, however, have been available on two enzymes, glutamic-oxalacetic transaminase (GOT) and lactic acid dehydrogenase