Lactate solid-state biosensor with multilayer of electrodeposited polymers for flow-injection clinical analysis
✍ Scribed by Tadeusz Krawczyñski vel Krawczyk; Marek Trojanowicz; Andrzej Lewenstam; Anna Moszczynska
- Book ID
- 103976147
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 870 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0956-5663
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✦ Synopsis
In the lactate biosensor, electrodeposited poly(o-phenylenediamine) serves as a convenient matrix for the immobilization of lactate oxidase, but does not provide sufficient discrimination from several interfering species present in physiological fluids. Their effect, however, can be eliminated by additional modification of the working Pt electrode with a bilayer of electrodeposited polypyrrole/polyphenol. Despite continued decrease in biosensor sensitivity, the newly developed three-layer solid-state biosensor was successfully applied in flow-injection determination of lactate in both undiluted and diluted human blood serum samples over a 10 day period. For the lactate concentration range 0.2-5.0 mM in several series of measurements the correlation coefficient values for comparison with photometric determination using a DuPont dimension clinical analyzer were between 0.96 and 0.99. The reproducibility measured for 1:10 diluted serum was 0.6%. The detection limit was estimated as 2 microM.