Lactate dehydrogenase — X — separation of human spermatozoa isoenzymes by extraction with detergents

Detection of activity and mass spectrome

Detection of activity and mass spectrometric identification of mouse liver carboxylesterase and aldehyde dehydrogenase separated by non-denaturing two-dimensional electrophoresis after extraction with detergents

✍ Youji Shimazaki; Takashi Manabe 📂 Article 📅 2005 🏛 Elsevier Science 🌐 English ⚖ 808 KB

To examine the activities and identity of enzymes associated with organelles such as microsomes and mitochondria, proteins from mouse liver were extracted using the non-ionic detergents Nonidet P-40 (NP-40), polyoxyethylene sorbitan monooleate (Tween 80), polyoxyethylene isooctylphenyl ester (Triton