Lack of short-term effects on the donor during continuous-flow selective mononuclear cell collection
✍ Scribed by Professor Peter Jacobs; Lucille Wood
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 258 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0733-2459
No coin nor oath required. For personal study only.
✦ Synopsis
In SO individuals. intensive harvesting of relatively pure mononuclear cell fractions from the peripheral circulation was carried out in 195 procedures. Serial collections from bone marrow donors (group 1: n = 35) or isolated procedures from volunteers (group 2: n = 15) were without morbidity. A median yield of 4.0 X lo9 mononuclear cells were recovered in a final volume of 104 nil of cell-rich plasma. for which 4,300 ml of venous blood was proccsscd in 107 minutes. In neither group were changes documented in donor white cell count or lymphocyte numbers. In group I , a statistically significant but clinically unimportant and transient fall occurred in the platelet count at the end of the 5-day intensive schedule. It is concluded that mononuclear fractions can efficiently be collectcd from normal donors without the development of relevant cell depletion.