Laccase induction in the white-rot fungusHeterobasidion annosum(Fr.) Bref. (Fomes annosusFr. Cooke)
✍ Scribed by Annegret Haars; Aloys Hüttermann
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 506 KB
- Volume
- 134
- Category
- Article
- ISSN
- 0302-8933
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✦ Synopsis
The laccase activity in the culture medium of Fomes annosus is greatly increased by exposure to lignosulfonate. This increase is due to de novo synthesis of the enzyme, as shown by density-labelling. However, intraceltular enzyme molecules, present before induction, are conserved for at least ten days after the addition of the inducer.
The surface of the fungal hyphae contains specific receptors for phenolic compounds. These receptors can be visualized with fluorescence-labelled lignosulfonate. They can be blocked with a synthetic phenolic macromolecule that is not degraded or taken up by the fungus; this macromolecule inhibits laccase induction by small phenols.